Single-Gene Knockout vs Double-Gene Knockdown and behavior

Single-Gene Knockout Collection Created

The technique and report on it is outside my field so I hope someone will comment on my perception of a similar approach in:

RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)”

The authors make claims in their abstract for a double gene knockdown approach combined with a.proboscis extension response (PER) assay in honey bees that involves olfactory conditioning of learning and memory in honeybees, which is detailed in the context of gustatory responses / behavior.

In my model, this links choices associated with food odors to their epigenetic effects on de novo creation of olfactory receptor genes and it also links those choices to  the metabolism of nutrients to species-specific pheromones, which control reproduction in species from microbes to man. I take this to mean that the double gene knock-down has greater explanatory power in the context of systems biology and speciation — if divergence of species is due to nutrient-dependent pheromone-controlled adaptive evolution.

However, if a case can be made for mutation-driven evolution, I wonder if the single gene knockout collection will prove to have greater explanatory power. The problem for me is that things like cell cycle control, metabolism, and antibiotic resistance appear to be nutrient-dependent and pheromone-controlled, not mutation-driven. Is there a model organism of mutation-driven thermodynamics and organism-level thermoregulation for comparison of what happens when cell lines contain a single mutated gene that is not controlled by reproduction? If not, does the fact that there is no model eliminate mutation-driven evolution from further consideration?

About James V. Kohl 1308 Articles
James Vaughn Kohl was the first to accurately conceptualize human pheromones, and began presenting his findings to the scientific community in 1992. He continues to present to, and publish for, diverse scientific and lay audiences, while constantly monitoring the scientific presses for new information that is relevant to the development of his initial and ongoing conceptualization of human pheromones. Recently, Kohl integrated scientific evidence that pinpoints the evolved neurophysiological mechanism that links olfactory/pheromonal input to genes in hormone-secreting cells of tissue in a specific area of the brain that is primarily involved in the sensory integration of olfactory and visual input, and in the development of human sexual preferences. His award-winning 2007 article/book chapter on multisensory integration: The Mind’s Eyes: Human pheromones, neuroscience, and male sexual preferences followed an award winning 2001 publication: Human pheromones: integrating neuroendocrinology and ethology, which was coauthored by disinguished researchers from Vienna. Rarely do researchers win awards in multiple disciplines, but Kohl’s 2001 award was for neuroscience, and his 2007 “Reiss Theory” award was for social science. Kohl has worked as a medical laboratory scientist since 1974, and he has devoted more than twenty-five years to researching the relationship between the sense of smell and the development of human sexual preferences. Unlike many researchers who work with non-human subjects, medical laboratory scientists use the latest technology from many scientific disciplines to perform a variety of specialized diagnostic medical testing on people. James V. Kohl is certified with: * American Society for Clinical Pathology * American Medical Technologists James V. Kohl is a member of: * Society for Neuroscience * Society for Behavioral Neuroendocrinology * Association for Chemoreception Sciences * Society for the Scientific Study of Sexuality * International Society for Human Ethology * American Society for Clinical Laboratory Science * Mensa, the international high IQ society