Single-cell level assay of protein biosynthesis and degradation

High-throughput detection of miRNAs and gene-specific mRNA at the single-cell level by flow cytometry

Excerpt: “We use this technique to show modulation of a microRNA critical for T-cell function, miR-155. We adapt this assay for simultaneous detection of mRNA and proteins by ImageStream technology.”

Reported as:

Technology breakthrough reveals cellular transcription process

My comment: Their assay simultaneously detects epigenetically-effected changes in the miRNA/mRNA balance and proteins, which link nutrient-uptake to the physical landscape of DNA in the organized genomes of species from microbes to man.At the single-cell level, they are beginning to assess how RNA-directed DNA methylation and RNA-mediated events link bio-physically constrained thermodynamic cycles of protein biosynthesis and degradation to “…translational and expressional effects in both normal and pathologic conditions.”

They have moved beyond what already is available to link metabolic networks and genetic networks in the context of pharmacogenomics, which enabled serious scientists to distinguish the difference between effects of mutations that perturb protein folding and RNA-mediated amino acid substitutions that stabilize DNA in organized genomes via fixation in the context of pheromone-controlled reproduction.

I had hoped that this breakthrough would link nutrient-dependent RNA-directed DNA methylation from RNA-mediated events and amino acid substitutions to cell type differentiation via epigenetic effects on the microRNA/messenger RNA balance in species from microbes to man. Predictably, that will be the next step forward via use of their technique, which indirectly offers yet another refutation of evolutionary theories about beneficial mutations.

For examples see: Nutrient-dependent/pheromone-controlled adaptive evolution: a model

Excerpt: “..the epigenetic ‘tweaking’ of the immense gene networks that occurs via exposure to nutrient chemicals and pheromones can now be modeled in the context of the microRNA/messenger RNA balance, receptor-mediated intracellular signaling, and the stochastic gene expression required for nutrient-dependent pheromone-controlled adaptive evolution.”

See also:

From Fertilization to Adult Sexual Behavior (1996)

Clinically Actionable Genotypes Among 10,000 Patients With Preemptive Pharmacogenomic Testing (2013)

About James V. Kohl 1308 Articles
James Vaughn Kohl was the first to accurately conceptualize human pheromones, and began presenting his findings to the scientific community in 1992. He continues to present to, and publish for, diverse scientific and lay audiences, while constantly monitoring the scientific presses for new information that is relevant to the development of his initial and ongoing conceptualization of human pheromones. Recently, Kohl integrated scientific evidence that pinpoints the evolved neurophysiological mechanism that links olfactory/pheromonal input to genes in hormone-secreting cells of tissue in a specific area of the brain that is primarily involved in the sensory integration of olfactory and visual input, and in the development of human sexual preferences. His award-winning 2007 article/book chapter on multisensory integration: The Mind’s Eyes: Human pheromones, neuroscience, and male sexual preferences followed an award winning 2001 publication: Human pheromones: integrating neuroendocrinology and ethology, which was coauthored by disinguished researchers from Vienna. Rarely do researchers win awards in multiple disciplines, but Kohl’s 2001 award was for neuroscience, and his 2007 “Reiss Theory” award was for social science. Kohl has worked as a medical laboratory scientist since 1974, and he has devoted more than twenty-five years to researching the relationship between the sense of smell and the development of human sexual preferences. Unlike many researchers who work with non-human subjects, medical laboratory scientists use the latest technology from many scientific disciplines to perform a variety of specialized diagnostic medical testing on people. James V. Kohl is certified with: * American Society for Clinical Pathology * American Medical Technologists James V. Kohl is a member of: * Society for Neuroscience * Society for Behavioral Neuroendocrinology * Association for Chemoreception Sciences * Society for the Scientific Study of Sexuality * International Society for Human Ethology * American Society for Clinical Laboratory Science * Mensa, the international high IQ society