Evolutionary theorists and evolutionary theists live under rocks

CRISPR/Cas: Not Just for DNA Anymore

Excerpt 1) “Unless you’ve been living under a rock the past two years, you’ve heard of CRISPR/Cas9, an RNA-guided nuclease that’s redefining synthetic biology and genome engineering.”

Excerpt 2) “The authors propose several additional applications as well, including… altering pre-mRNA splicing, and transcript tracking in live cells.”

My comment: Are you an evolutionary theorist or evolutionary theist who has continued to ignore everything known about RNA-mediated events that link nutrient-uptake to altered pre-mRNA splicing and cell type differentiation via amino acid substitutions? If so, you are among many who appear to have been living under a rock for nearly two decades.

In 1996, we wrote: “Small intranuclear proteins also participate in generating alternative splicing techniques of pre-mRNA and, by this mechanism, contribute to sexual differentiation in at least two species, Drosophila melanogaster and Caenorhabditis elegans (Adler and Hajduk, 1994; de Bono, Zarkower, and Hodgkin, 1995; Ge, Zuo, and Manley, 1991; Green, 1991; Parkhurst and Meneely, 1994; Wilkins, 1995; Wolfner, 1988). That similar proteins perform functions in humans suggests the possibility that some human sex differences may arise from alternative splicings of otherwise identical genes.”

That explanation of how sex differences in cell type differentiation arise has since been extended to cell type differentiation in all cells of all individuals of all species via conserved molecular mechanisms of RNA-directed DNA methylation. RNA-mediated events link the epigenetic landscape to the physical landscape of DNA via amino acid substitutions that differentiate cell types.

No biologically-based evolutionary events have been described. None will be. Mutations perturb the protein folding that is required for ecological variation to lead to ecological adaptations. Mutations cannot link natural selection to the evolution of biodiversity because protein folding is biophysically constrained (e.g., by the laws of physics).

About James V. Kohl 1307 Articles
James Vaughn Kohl was the first to accurately conceptualize human pheromones, and began presenting his findings to the scientific community in 1992. He continues to present to, and publish for, diverse scientific and lay audiences, while constantly monitoring the scientific presses for new information that is relevant to the development of his initial and ongoing conceptualization of human pheromones. Recently, Kohl integrated scientific evidence that pinpoints the evolved neurophysiological mechanism that links olfactory/pheromonal input to genes in hormone-secreting cells of tissue in a specific area of the brain that is primarily involved in the sensory integration of olfactory and visual input, and in the development of human sexual preferences. His award-winning 2007 article/book chapter on multisensory integration: The Mind’s Eyes: Human pheromones, neuroscience, and male sexual preferences followed an award winning 2001 publication: Human pheromones: integrating neuroendocrinology and ethology, which was coauthored by disinguished researchers from Vienna. Rarely do researchers win awards in multiple disciplines, but Kohl’s 2001 award was for neuroscience, and his 2007 “Reiss Theory” award was for social science. Kohl has worked as a medical laboratory scientist since 1974, and he has devoted more than twenty-five years to researching the relationship between the sense of smell and the development of human sexual preferences. Unlike many researchers who work with non-human subjects, medical laboratory scientists use the latest technology from many scientific disciplines to perform a variety of specialized diagnostic medical testing on people. James V. Kohl is certified with: * American Society for Clinical Pathology * American Medical Technologists James V. Kohl is a member of: * Society for Neuroscience * Society for Behavioral Neuroendocrinology * Association for Chemoreception Sciences * Society for the Scientific Study of Sexuality * International Society for Human Ethology * American Society for Clinical Laboratory Science * Mensa, the international high IQ society